2017 MAP Conference: Detecting Mycobacterium avium subspecies paratuberculosis (MAP) in Crohn’s Disease Patients

by Dr. Adrienne McNees

Dr. McNees is an associate of Dr. David Graham in the Department of Molecular Virology and Microbiology at the Baylor College of Medicine in Houston, TX. She talks about their methods to detect MAP in human subjects using culture and PCR, and discusses their results. MAP was detected in 70% of Crohn’s disease patients and 48.9% of controls. When the disease is in the colon, more cases are MAP-positive.

Video Presentation

 

Q&A Session

Have you DNA sequenced all of the positive results?
How many colonies of MAP do you see?
How do you account for the high incidence of MAP in controls?
Were blood samples cultured aerobically for other organisms?
Is IS900 unique and specific for MAP?

 

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